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KMID : 1134820190480121323
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 12 p.1323 ~ p.1229
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Chrysophanol Suppressed the Level of Pro-Inflammatory Cytokines Induced by LPS in Raw 264.7 Macrophage Cells
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Pan Yanni
Park Eui-Seong
Hong Geun-Hye
Lim Yaung-Iee
Park Kun-Young
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Abstract
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The level of pro-inflammatory cytokines was markedly suppressed by chrysophanol in lipopolysaccharide (LPS)-treated Raw 264.7 macrophage cells. Chrysophanol had no toxic effect on the Raw 264.7 cells at the treatment concentrations ranging from 30 to 60 μM. Sixty μM (High) chrysophanol exhibited the highest protection against the LPS effect on the Raw 264.7 cells, as was determined by MTT assay. Thirty μM (Low) and 60 μM concentrations of chrysophanol significantly decreased the production of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6, and IL-10 in the LPS-treated Raw 264.7 cells as compared to that of LPS treatment only. LPS＋Low and LPS＋High also significantly suppressed the mRNA expressions of the pro-inflammatory cytokines TNF-α, IL-1β, INF-γ, and COX-2 compared to that of LPS treatment only. Moreover, LPS＋Low and LPS＋High significantly decreased the protein expressions of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, and also the inflammation related proteins of iNOS and COX-2 compared to that of LPS treatment only in the cells. According to our results, chrysophanol, which is derived from plants and especially curly dock, showed suppressive effects by inhibiting the inflammatory effects induced by LPS in Raw 264.7 cells by regulating pro-inflammatory cytokines and inflammation related enzymes. These results indicated that chrysophanol may possibly be used to treat inflammatory diseases and it is perhaps a marker of anti-inflammatory functions in plants.
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KEYWORD
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chrysophanol, anti-inflammation, LPS, pro-inflammatory cytokines, Raw 264.7 cells
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